抗菌肽JH-3的抑菌活性及其稳定性分析

doi:10.11843/j.issn.0366-6964.2016.02.020

Abstract

Abstract:

The aim of the present study was to modify antimicrobial peptide P3 from bovine hemoglobin on amino acid，and to know the antibacterial activity and stability of the antibacterial peptide JH-3 modified based on P3.The minimum inhibitory concentrations (MIC) of JH-3 against several bacteria were detected with the method of serial dilution.The morphology changes of bacterial and fungus were observed by scanning electron microscope (SEM).The hemolytic activity，thermal stability，acid base stability and salt stability of the antibacterial peptide JH-3 were determined.The results showed that the MIC of JH-3 against gram positive bacteria (Staphylococcus aureus， Streptococcus suis 2 type) was between 6.25-25 μg•mL^-1.The MIC against Gram negative bacteria (Escherichia coli， Salmonella choleraesuis， Haemophilus parasuis， Actinobacillus pleuropneumoniae) was between 3.125-50 μg•mL^-1.The MIC against fugus (Monilia albicans) was 12.5 μg•mL^-1.Shrinkage and holes appeared on the surface of treated E.coli cells.Shrinkage and incomplement presented on the cell surface of S.Aureus.And M.albican showed sunken，protruding and crack.Hemolytic rate of JH-3 was only 12.84% at 312.5 μg•mL^-1 (6-100 times MIC).At the condition of 75-100 ℃，80-100 mmol•L^-1 NaCl，pH 4 and pH 10，the antimicrobial activity of JH-3 was not changed significantly.These findings provided theoretical basis for the clinical application of antimicrobial peptide JH-3，and antibacterial peptide JH-3 has the potential as the new antibacterial drugs instead of antibiotics.

CLC Number:

S852.4

WEI Xiao-xiao，HANG Bo-lin，MA Cui，XIA Yi-he，ZHANG Bing-qing，WANG Qing，XU Yan-zhao，HU Jian-he. Analysis of Antimicrobial Activity and Stability of Antibacterial Peptide JH-3[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(2): 361-366.

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Analysis of Antimicrobial Activity and Stability of Antibacterial Peptide JH-3

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